StrepTactin Sepharose High Per
产品名称： StrepTactin Sepharose High Per
英文名称： StrepTactin Sepharose High Per
产品编号： 28-9356-00
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
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| 28-9356-00 | StrepTactin Sepharose High Per 50ML |
| 28-9805-82 | MembraneProteinPurificationKit |
| 17-5468-15 | HiScreen Capto SP ImpRes |
| 17-5470-15 | HiScreen Capto Q ImpRes |
| 17-5474-15 | HiScreen MabSelect SuRe LX |
StrepTactin Sepharose High Performance/StrepTrap HP
- Highly pure Strep(II)-tagged1 recombinant proteins eluted in concentrated form and small volumes.
- StrepTrap HP 1 ml and 5 ml columns prepacked with StrepTactin™ Sepharose™ High Performance give convenient, time-saving operation and reproducible results.
- Physiological conditions and mild elution preserves the activity of the target protein.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Easy scale-up and easily regenerated with 0.5 M NaOH.
- Convenient use with a peristaltic pump, or chromatography system such as ÄKTAdesign™.
StrepTactin Sepharose High Performance/StrepTrap HP
Technical Information
-tagged recombinant proteins to high purities in concentrated forms and small volumes.'))
StrepTactin Sepharose High Performance and StrepTrap HP 1 ml and 5 ml columns for purification of Strep(II)-tagged recombinant proteins to high purities in concentrated forms and small volumes.
StrepTrap™ HP is supplied ready to use for convenient purification of Strep(II)-tagged recombinant proteins by affinity chromatography. These columns are prepacked with StrepTactin Sepharose High Performance. This robust and stable Sepharose based medium results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Regeneration of StrepTactin Sepharose High Performance and StrepTrap HP is fast and easily performed with 0.5 M NaOH.
 purification runs on the same StrepTrap HP 1 ml column including regeneration with 0.5 M NaOH between each run. For SDS-PAGE analysis and recovery data, see next page.'))
Six repetitive GAPDH-Strep(II) purification runs on the same StrepTrap HP 1 ml column including regeneration with 0.5 M NaOH between each run. For SDS-PAGE analysis and recovery data, see next page.
 of the six repetitive purifications runs in the regeneration study shows excellent reproducibility.'))
SDS-PAGE (reducing conditions) of the six repetitive purifications runs in the regeneration study shows excellent reproducibility.
)
Recovery in the eluted fractions was retained over six purification runs.
| COMPATABILITY GUIDE - Compatibility of StrepTactin Sepharose High Performance with different additives* | |
| Reagent | Concentration |
| Reducing agents | |
| DTT | 50 mM |
| b-mercaptoethanol | 50 mM |
| Nonionic detergents | |
| C8E4, Octyltetraoxyethylene | max. 0.88% |
| C10E5, Decylpentaoxyethylene | 0.12% |
| C10E6 | 0.03% |
| C12E8 | 0.01% |
| C12E9, Dodecyl nonaoxyethylene (Thesit) | 0.02% |
| Decyl-b-D-maltoside | 0.35% |
| N-dodecyl-b-D-maltoside | 0.01% |
| N-nonyl-b-D-glucopyranoside | 0.20% |
| N-octyl-b-D-glucopyranoside | 2.34% |
| Triton X-100 | 2% |
| Tween 20 | 2% |
| Ionic detergents | |
| N-lauryl-sarcosine | 2% |
| 8-HESO; N-octyl-2-hydroxy-ethylsulfoxide | 1.32% |
| SDS, Sodium-N-dodecyl sulfate | 0.10% |
| Zwitterionic detergents | |
| CHAPS | 0.10% |
| DDAO, N-decyl-N,N-dimethylamine-N-oxide | 0.03% |
| LDAO, N-dodecyl-N,N-dimethylamine-N-oxide | 0.13% |
| Others | |
| Ammonium sulfate, (NH4)2SO4 | 2 M |
| CaCl2 | max. 1 M |
| EDTA | 50 mM |
| Guanidine | max. 1 M |
| Glycerol | max. 25%† |
| Imidazole | 500 mM‡ |
| MgCl2 | 1 M |
| Urea | max. 1 M |
| NaCl | 5 M |
| These reagents have been successfully tested for the purification of, for example, GAPDH-Strep(II) with concentrations up to those listed. For reagents not marked with 'max', higher concentrations may be possible. Binding depends on the sterical accessibility of the Strep(II) tag and therefore varies from protein to protein. * Data kindly provided by IBA GmbH, Germany, the manufacturer and intellectual property owner of the StrepTactin ligand. † Yield may decrease. ‡ 500 mM imidazole in sample tested by GE Healthcare.
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| TECHNICAL SPECIFICATIONS | |
| Matrix | Rigid, highly cross-linked 6% agarose |
| Average particle size | 34 μm |
| Ligand | StrepTactin |
| Dynamic binding capacity1 | Approx. 6 mg Strep(II)-tagged protein/ml medium |
| Compatibility | Stable in all commonly used buffers, 0.5 M NaOH (regeneration and cleaning), reducing agents, denaturants, and detergents, see table on next page for more information |
| pH stability | > 7 |
| Storage | 4°C to 8°C in 20% ethanol |
| StrepTrap HP | |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1ml); 1.6 × 2.5 cm (5 ml) |
| Recommended flow rate2 | 1 ml/min (1 ml); 5 ml/min (5 ml) |
| Max. flow rate2 | 4 ml/min (1 ml); 20 ml/min (5 ml) |
| Max. pressure2 | 0.3 MPa, 3 bar |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 8°C |
| 1 Binding capacity is protein dependent. Dynamic binding capacity was tested with GAPDH-Strep(II), Mr 37 400) and MBP-bGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution) 2 H20 at room temperature |
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