淋巴细胞功能关联抗原1α(LFA1α)重组蛋白

YB572Ra011
Recombinant Lymphocyte Function Associated Antigen 1 Alpha (LFA1a)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
淋巴细胞功能关联抗原1α(LFA1α)重组蛋白[ PROPERTIES ]
Residues: Asn809~His1066 (Accession # P20701),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
淋巴细胞功能关联抗原1α(LFA1α)重组蛋白Predicted isoelectric point: 5.5
Predicted Molecular Mass: 32.7kDa
Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
淋巴细胞功能关联抗原1α(LFA1α)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGS S HHHHH H SSG LVP R GS H MASM TGGQ QM GRGSEF - NL EE DAYWVQ LD
LHFPPGLSFR KVEMLKPHSQ IPVSCEELPE ESRLLSRALS CNVSSPIFKA GHSVALQMMF
NTLVNSSWGD SVELHANVTC NNEDSDLLED NSATTIIPIL YPINILIQDQ EDSTLYVSFT
PKGPKIHQVK HMYQVRIQPS IHDHNIPTLE AVVGVPQPPS EGPITHQWSV QMEPPVPCHY
EDLERLPDAA EPCLPGALFR CPVVFRQEIL VQVIGTLELV GEIEASSMFS LCSSLSISFN
SSKHFH
[ REFERENCES ]
1. Larson R.S., et al. (1989) J. Cell Biol. 108:703-712. 2. Nueda A., et al. (1993) J. Biol. Chem. 268:19305-19311. 3. Cornwell R.D., et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:4221-4225. 4. Qu A., Leahy D.J. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:10277-10281.